• What Is icsi?
  • icsi Procedure
  • Why Choose Kasturi icsi ?
  • Do's And Dont's

A specialized method of in vitro fertilization (IVF) called intracytoplasmic sperm injection (ICSI) is used to treat severe cases of male-factor infertility. In ICSI, a single sperm is directly injected into a developed egg.

At City Fertility, we choose sperm using a process called Sperm Slow. Hyaluronan (HA), a substance in this medium that binds sperm more likely to have healthy DNA, enables the selection of these bound sperm for injection. The embryologists are using more mature, higher-quality sperm by choosing those that are bound to HA and using them for ICSI. Please be aware that while utilizing HA is a common practice, it may not always be appropriate depending on the specific situation.

Please keep in mind that anybody can experience infertility, and while it can be a difficult time, the good news is that our specialists can help you explore the possibilities so you can realize your dream of becoming a parent.


The good news is that our professionals can help you explore the alternatives available to realize your desire of becoming a parent. Please keep in mind that anybody can be affected by infertility, and while it can be a difficult experience, anyone can be affected by it.

Step one: Stimulation of the ovaries

You will take injectable drugs for roughly 8 to 14 days during the stimulation phase of an ICSI cycle to stimulate the ovaries to generate eggs. The drugs are made up of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which the body naturally produces. You will visit the clinic seven to eight times throughout this phase for morning monitoring, which enables the medical staff to monitor the development of your cycle and alter medication dosages as necessary. The last stage of the stimulation phase of treatment is the trigger injection. You will either receive a human chorionic gonadotropin (hCG) or a buserelin trigger, depending on your specific procedure. This injection initiates ovulation and aids in the maturation of the developing eggs. The doctor must execute the egg retrieval before the anticipated period of ovulation, thus timing is crucial in this case.

Step two: Egg retrieval

At Glasgow Royal Fertility Clinic, your egg retrieval operation will be handled by a medical professional. Before the procedure on the morning of your egg retrieval, a doctor will meet with you to go over your protocol. Additionally, an anesthetist will meet with you to go over your medical history and deliver the intravenous fluid that will put you to sleep prior to the surgery starts.

A member of the embryology team will visit to collect the sperm if you are utilizing a fresh sperm sample. The embryologist will confirm those specifics with you if you're using donor sperm that was previously obtained frozen or another type of sperm sample. The sperm will be cleaned and prepared at our andrology laboratory so that only the healthiest sperm are combined with the eggs for conception (after the doctor performs the egg retrieval).

How to get the eggs: Egg retrieval takes 20 to 30 minutes in total. The doctor will insert a needle into each ovary during the process to drain the fluid from each follicle that contains an egg. During the process, the doctor uses an ultrasound to see where to direct the needle. You'll be able to walk out on your own after a 30-minute recovery period. After the surgery, it is crucial that a responsible adult drive you home because it is dangerous to drive after having anesthesia. The person who is driving you doesn't need to stay at our office while you have your operation; instead, they should plan on returning in around 3 hours.

Step three: Fertilisation

The embryologist will prepare the eggs and sperm after the egg retrieval. Under a microscope, the embryologist performing PGD will inject one single sperm into each developed egg. Therefore, PGD can be performed using very little sperm.

Step four: Embryo development

Development of the embryo starts after fertilization. The embryos will be placed within our Embryoscopes after initial fertilization so they can continue to develop fully undisturbed. Over the next 5 to 6 days, an embryologist analyses each developing embryo. A two to four-cell embryo on day two and a six to eight-cell embryo on day three are the desired stages of development. Rapid cell division continues after the eight-cell stage, and by day 5 or 6, the embryo has reached the so-called blastocyst stage. To give you the best chance of success, your doctor will transfer the best possible embryo(s).

Step five: Embryo transfer

The embryo transfer is a quick process that takes only a few minutes to finish. Neither anesthesia nor recuperation time is required. Your nurse will inform you and give instructions on how to prepare and when to arrive when she arranges your transfer. For the procedure, you must have a full bladder since a full bladder ensures clear visibility of the uterine lining and accurate positioning of the embryos. It's crucial to consume the proper amount of liquid 30–40 minutes beforehand. With the doctor, you will discuss your cycle and the recommended number of embryos for transfer. The embryologist will confirm your last name and the number of embryos to be transferred once you are in the treatment room. The doctor will put the transfer catheter into the uterus and push the embryo through with a small amount of fluid after the embryologist loads the catheter with the embryo(s) at the embryology lab. During the surgery, external abdomen ultrasonography gives the doctor visual guidance on a monitor. The catheter will be gradually removed by the doctor after the embryo has been transferred. The embryologist will next check the catheter in the lab under a microscope to confirm that the embryo was really released because the embryo is invisible to the naked eye. Before it's time for the pregnancy test, the nurse will give you instructions for the ensuing two weeks.

Step six: The pregnancy test

You'll test for pregnancy two weeks following the embryo transfer. Because it examines the beta chain of the hCG hormone released by the developing embryo, this test is usually referred to as a "beta." We allow you to use a urine pregnancy test in the privacy of your own home, and you then report the results so we can choose the next course of action.

  • Skilled Staff: Kasturi has a committed staff of clinicians and embryologists who work to offer the best-individualized care to all infertile couples while keeping up with the most recent and cutting-edge technologies.
  • Modern infrastructure: In Kasturi, there is an OT complex and a clean room for assisted reproductive technologies.
  • Options for specialized testing and treatment of male infertility.
  • A comfortable atmosphere and surroundings.
  • An IVF lab with bio-clad, anti-microbial walls in a clean room.

Key Success factors

  • Top Specialist Team with Nearly Zero Attrition
  • Comprehensive domain knowledge in the field of fertility.
  • A fixation with excellence.
  • Trait that sets trends.
  • Undiluted, unwavering focus on and expertise in the field of fertility.
  • A Treasure Chest of Specialties in Fertility
  • Entrepreneurial leadership that is frugal.
  • Innovative Projects.
  • Ethical Conduct.
  • NU Team.
  • Approach with the head, shoulders, and feet on the ground.
  • A growth strategy based on cautious optimism.
  • High Governance Standards and Strict Compliance.
  • Enterprise and Infrastructure Able to Support Multi Centric Operations.


The list of dos and don'ts for someone undergoing ICSI is often the same as for someone undergoing an IVF cycle. To name a few:


Keep an eye on what you eat and drink because diet affects sperm production and count. Therefore, it's crucial that they visit their doctor at least three months before the appointment and get a food plan that will help them increase their fertility.

Focus on proteins: Protein is a superfood that has been shown to boost sperm quality as well as general health. This will guarantee that on the day your process starts, you can provide the greatest sample you possibly can.


Reduce alcohol consumption: Avoiding alcohol for at least three months prior to the desired IVF treatment is one of the recommended ways to speed up the process.

Give up smoking. Numerous scientific studies and data demonstrate that smoking has a detrimental effect on every component of the human body. The most severe and frequently irreversible effects, however, are those on one's reproductive health. While quitting smoking prior to therapy may seem like a little step, it does benefit.

Dr. M. Kalpana (DNB, OBGYN, Obstetrician & Gynaecologist Fertility Specialist Fetal Medicine Specialist)

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